Evaluation of Three Cell Culture Systems as Substrates for Influenza Virus Assay
نویسندگان
چکیده
منابع مشابه
Culture and Assay Systems Used for 3D Cell Culture
Mammalian cell culture has served as an invaluable tool in cell biology for several decades. Mono layers of adherent cells grown on flat and rigid two-dimensional (2D) substrates, such as polysty rene or glass, have evolved as the mainstay in conventional cell culture systems. Two-dimensional cell culture studies have played a pivotal role in furthering our understanding of developmental biol...
متن کاملCell culture systems for hepatitis C virus.
Due to the obligatory intracellular lifestyle of viruses, cell culture systems for efficient viral propagation are crucial to obtain a detailed understanding of the virus-host cell interaction. For hepatitis C virus (HCV) the development of permissive and authentic culture models continues to be a challenging task. The first efforts to culture HCV had limited success and range back to before th...
متن کاملOptimization of Microcarrier-based MDCK-SIAT1 Culture System for Influenza Virus Propagation
Introduction: The preparation of seasonal influenza virus vaccines and especially its large-scale production requirement after the emergence or reemergence of a pandemic will need an alternative host cell system due to current suboptimal methods and the insufficiency of embryonated chicken eggs needed for producing them. In response to the vital and increasing demand for alternative means for ...
متن کاملEffects of different culture media on optimization of primary neuronal cell culture for in vitro models assay
Background: In vitro model studies are becoming increasingly popular for experimental research designs. They include isolation and expansion of cells of a particular tissue, such as the nervous tissue which contributes to understanding the underlying mechanisms in many pathologies. It enables the scrutinization of intracellular signaling pathways responsible for cell death. OBJECTIVES: In the ...
متن کاملCulture and Assay Systems Utilized for Cancer Stem Cell Research
Advances in stem cell biology over the last two decades have provided new insights into cancer biology. Tumors show marked heterogeneity in morphology, proliferation rates, genetic lesions and therapeutic response. Thus, not all tumor cells are equal. This heterogeneity is even seen in an individual tumor that is clonal. The cellular and molecular basis for tumor heterogeneity represents a fund...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1970
ISSN: 0003-6919
DOI: 10.1128/aem.19.4.580-582.1970